Abstract
Objectives: Mutations/deletions affecting the TP53 gene are considered an independent marker predicting a poor prognosis for patients with diffuse large B-cell lymphoma (DLBCL). A cohort within a genetically isolated population was investigated for p53 mutation/deletion status. Methods:Deoxyribonucleic acid (DNA) samples were extracted from 23 paraffin-embedded blocks obtained from DLBCL patients, and subjected to polymerase chain reaction (PCR) amplification and sequencing of exons 4–9 of the p53 gene. Results: While 35% of patients analysed displayed allelic deletions (P<0.01), immunohistochemical analysis revealed a mutation rate of 69.5%. It is noteworthy that the rate of p53 mutations/deletions in this small cohort was found to be higher than that previously reported in the literature. Interestingly, patients with p53 mutations displayed a better overall survival when compared to those without. The survival of patients treated with rituximab-containing combination chemotherapy was significantly better than those who did not receive rituximab (P <0.05). Furthermore, a modelling analysis of the deleted form of p53 revealed a huge structural change affecting the DNA-binding domain. Conclusion: The TP53 mutation/deletion status plays a role in mechanism(s) ruling the pathogenesis of DLBCL and may be useful for stratifying patients into distinct prognostic subsets.
Publication details
- DOI
- 10.18295/2075-0528.1546
- Journal
- Sultan Qaboos University Medical Journal, 14(1), 50-58
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY-ND 4.0
Cite this article
APA 7
Tamimi, Y., Al-Harthy, S., Al Kindi, I., Mohammed, Babiker, H., Al-Moundhri, M., & Burney, I. (2025). The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma. Sultan Qaboos University Medical Journal, 14(1), 50-58. https://doi.org/10.18295/2075-0528.1546
MLA 9
Tamimi, Yahya, et al. "The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma." Sultan Qaboos University Medical Journal, vol. 14, no. 1, 2025, pp. 50-58. https://doi.org/10.18295/2075-0528.1546.
Chicago (author–date)
Tamimi, Yahya, Sheikha Al-Harthy, Ibrahim Al Kindi, Mohammed, Hamza Babiker, Mansour Al-Moundhri, and Ikram Burney. 2025. "The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma." Sultan Qaboos University Medical Journal 14 (1): 50-58. https://doi.org/10.18295/2075-0528.1546.
Harvard
Tamimi, Y., Al-Harthy, S., Al Kindi, I., Mohammed, Babiker, H., Al-Moundhri, M. and Burney, I. (2025) 'The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma', Sultan Qaboos University Medical Journal, 14(1), pp. 50-58. doi:10.18295/2075-0528.1546.
Vancouver
Tamimi Y, Al-Harthy S, Al Kindi I, Mohammed, Babiker H, Al-Moundhri M, et al. The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma. Sultan Qaboos University Medical Journal. 2025;14(1):50-58. doi:10.18295/2075-0528.1546
IEEE
Y. Tamimi, S. Al-Harthy, I. Al Kindi, Mohammed, H. Babiker, M. Al-Moundhri, and I. Burney, "The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma," Sultan Qaboos University Medical Journal, vol. 14, no. 1, pp. 50-58, 2025, doi: 10.18295/2075-0528.1546.