Abstract
Objectives: Induced dilated cardiomyopathy is the main limitation of the anti-cancer drug doxorubicin, which causes oxidative stress and cardiomyocyte death. As ozone therapy can activate the antioxidant systems, this study aimed to investigate the therapeutic efficacy of ozone-oxidative preconditioning against doxorubicin-induced cardiotoxicity. Methods: The study was carried out from September 2013 to January 2014. Sprague-Dawley rats were randomly distributed in the following treatment groups: Group 1 were treated with 2 mg/kg intraperitoneal (i.p.) of doxorubicin twice a week for 50 days; Group 2 were treated with 0.3 mg of ozone/oxygen mixture at 50 μg/ mL of ozone per 6 mL of oxygen by rectal insufflation and then treated with doxorubicin; Group 3 were treated as Group 2 but only with the oxygen, and Group 4 were treated with oxygen first, and then with sodium chloride i.p. as the control group. Results: The results showed that ozone therapy preserved left ventricle morphology which was accompanied by a reduction of serum pro-brain natriuretic peptide levels. The cardioprotective effects of ozoneoxidative preconditioning were associated with a significant increase (P <0.05) of antioxidant enzymes activities and a reduction of lipid and protein oxidation (P <0.05). Conclusion: Ozone-oxidative preconditioning prevents doxorubicin-induced dilated cardiomyopathy through an increase of antioxidant enzymes and a reduction of oxidised macromolecules. This establishes the background for future studies to determine if ozone therapy can be used as a complementary treatment for attenuating doxorubicin-induced cardiotoxicity in cancer patients.
Publication details
- DOI
- 10.18295/2075-0528.1597
- Journal
- Sultan Qaboos University Medical Journal, 14(3), 342-348
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY-ND 4.0
Cite this article
APA 7
Delgado-Roche, L., Hernández-Matos, Y., Morejón, E. A., Á., D., González, M. R., & Martίnez-Sánchez, G. (2025). Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats. Sultan Qaboos University Medical Journal, 14(3), 342-348. https://doi.org/10.18295/2075-0528.1597
MLA 9
Delgado-Roche, Livan, et al. "Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats." Sultan Qaboos University Medical Journal, vol. 14, no. 3, 2025, pp. 342-348. https://doi.org/10.18295/2075-0528.1597.
Chicago (author–date)
Delgado-Roche, Livan, Yanet Hernández-Matos, Emilio A. Morejón, Dalia Á., Maité R. González, and Gregorio Martίnez-Sánchez. 2025. "Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats." Sultan Qaboos University Medical Journal 14 (3): 342-348. https://doi.org/10.18295/2075-0528.1597.
Harvard
Delgado-Roche, L., Hernández-Matos, Y., Morejón, E. A., Á., D., González, M. R. and Martίnez-Sánchez, G. (2025) 'Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats', Sultan Qaboos University Medical Journal, 14(3), pp. 342-348. doi:10.18295/2075-0528.1597.
Vancouver
Delgado-Roche L, Hernández-Matos Y, Morejón EA, Á. D, González MR, Martίnez-Sánchez G. Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats. Sultan Qaboos University Medical Journal. 2025;14(3):342-348. doi:10.18295/2075-0528.1597
IEEE
L. Delgado-Roche, Y. Hernández-Matos, E. A. Morejón, D. Á., M. R. González, and G. Martίnez-Sánchez, "Ozone-Oxidative Preconditioning Prevents Doxorubicin-induced Cardiotoxicity in Sprague-Dawley Rats," Sultan Qaboos University Medical Journal, vol. 14, no. 3, pp. 342-348, 2025, doi: 10.18295/2075-0528.1597.