Abstract
Objectives: The present study was aimed to investigate the α-amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn (MeAc). Methods: Methanolic extract of Amaranthus caudatus was screened for α-amylase inhibition activity by CNPG3 method (2-chloro-p-nitrophenyl-α-D-maltotrioside) and antioxidant activity was evaluated by 1,1-diphenyl-2-picryl-hydrazile (DPPH) free radical scavenging, superoxide dismutase (SOD) scavenging, hydroxyl free radical scavenging, nitric oxide (NO) radical scavenging, and 2.2’-azinobis-3-ethylbenzothiazole-6-sulfonic acid (ABTS) radical scavenging assays. MeAc was also screened for non enzymatic hemoglycosylation. Results: The methanolic extract of Amaranthus caudatus showed potent α-amylase inhibition activity (IC50 19.233 µg/ml). MeAc showed significant antioxidant activity in all the in vitro antioxidant models. Furthermore, the MeAc was found to be extremely effective in scavenging ABTS radical activity (IC50 48.75±1.1 µg/ml) when compared to DPPH (IC50 77.5±0.4 µg/ml), SOD (IC50 62.5±2.1 µg/ml), hydroxyl (IC50 88.50±1.8 µg/ml) and NO (IC50 67.5±2.2 µg/ml) scavenging activity. Conclusions: The methanolic extract of A. caudatus showed potent α-amylase inhibition and antioxidant activities.
Keywords
Publication details
- DOI
- 10.5001/omj.2011.40
- Journal
- Oman Medical Journal
- Publisher
- Oman Medical Specialty Board
- Open access
- Gold open access
Cite this article
APA 7
BS, A. K., K, L., KN, J., D, S. S., VB, N. S., Khan, S., & C, V. (2011). In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn. Oman Medical Journal. https://doi.org/10.5001/omj.2011.40
MLA 9
BS, Ashok Kumar, et al. "In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn." Oman Medical Journal, 2011. https://doi.org/10.5001/omj.2011.40.
Chicago (author–date)
BS, Ashok Kumar, Lakshman K, Jayaveera KN, Sheshadri Shekar D, Narayan Swamy VB, Saleemulla Khan, and Velumurga C. 2011. "In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn." Oman Medical Journal. https://doi.org/10.5001/omj.2011.40.
Harvard
BS, A. K., K, L., KN, J., D, S. S., VB, N. S., Khan, S. and C, V. (2011) 'In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn', Oman Medical Journal. doi:10.5001/omj.2011.40.
Vancouver
BS AK, K L, KN J, D SS, VB NS, Khan S, et al. In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn. Oman Medical Journal. 2011. doi:10.5001/omj.2011.40
IEEE
A. K. BS, L. K, J. KN, S. S. D, N. S. VB, S. Khan, and V. C, "In Vitro α-Amylase Inhibition and Antioxidant Activities of Methanolic Extract of Amaranthus Caudatus Linn," Oman Medical Journal, 2011, doi: 10.5001/omj.2011.40.