Abstract
Marine sources have been known to yield novel compounds with a wide range of bioactivity with various commercial applications. In this study, the abilities of bacteria isolated from eight marine organisms to produce α-amylase were examined. All eight organisms were found to harbor amylase producing bacteria. Two bacterial species isolated from the green alga Ulva rigida and the sponge Mycale sp. were further identified and their α-amylases were purified and characterized. The bacterial species isolated from U. rigida and Mycale sp. were identified by DNA sequencing as Cellulosimicrobium sp. and Demequina sp., respectively. Cellulosimicrobium sp. obtained maximum cell growth and amylase production at 29.C and in the presence of lactose as a carbon source. Optimal cell growth and amylase production by Demequina sp. was observed at 35.C. While lactose enhanced cell growth of Demequina sp., maximum amylase production was found when fructose and glycerol were the available sources of carbon. Both strains grew better in the presence of tryptone, whilst peptone stimulated amylase production. Maximal cell growth and amylase production by both of the strains was found at a medium salinity of 3% NaCl.
Keywords
Publication details
- DOI
- 10.24200/jams.vol20iss0pp8-15
- Journal
- Journal of Agricultural and Marine Sciences, 20, 8
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY 4.0
Cite this article
APA 7
Al-Naamani, L. S., Dobretsov, S., Al-Sabahi, J. N., & Soussi, B. (2015). Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms. Journal of Agricultural and Marine Sciences, 20, 8. https://doi.org/10.24200/jams.vol20iss0pp8-15
MLA 9
Al-Naamani, Laila Salim, et al. "Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms." Journal of Agricultural and Marine Sciences, vol. 20, 2015, pp. 8. https://doi.org/10.24200/jams.vol20iss0pp8-15.
Chicago (author–date)
Al-Naamani, Laila Salim, Sergey Dobretsov, Jamal Nasser Al-Sabahi, and B. Soussi. 2015. "Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms." Journal of Agricultural and Marine Sciences 20: 8. https://doi.org/10.24200/jams.vol20iss0pp8-15.
Harvard
Al-Naamani, L. S., Dobretsov, S., Al-Sabahi, J. N. and Soussi, B. (2015) 'Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms', Journal of Agricultural and Marine Sciences, 20, pp. 8. doi:10.24200/jams.vol20iss0pp8-15.
Vancouver
Al-Naamani LS, Dobretsov S, Al-Sabahi JN, Soussi B. Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms. Journal of Agricultural and Marine Sciences. 2015;20:8. doi:10.24200/jams.vol20iss0pp8-15
IEEE
L. S. Al-Naamani, S. Dobretsov, J. N. Al-Sabahi, and B. Soussi, "Identification and characterization of two amylase producing bacteria Cellulosimicrobium sp. and Demequina sp. isolated from marine organisms," Journal of Agricultural and Marine Sciences, vol. 20, pp. 8, 2015, doi: 10.24200/jams.vol20iss0pp8-15.