Abstract
Objective and Method – To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3•– end of a primer to amplify a 268 bp (base pair) region of the human •–globin gene using different annealing temperatures (45 to 65•C). Results – The primer with the G/T mismatch was as efficient as the normal primer (G/C match) in the amplification of a 268 bp product at all temperatures tested. However, the primers having G/A or G/G mismatches at the 3'- end did not produce any specific polymerase chain reaction (PCR) fragment at all the annealing temperatures used, except a barely detectable 268 bp product for the G/G mismatch at 45 and 50•C. Conclusion – We conclude that our PCR system was refractory to amplification when one of the primers contained a G/A or G/G mismatch at the 3•–end with template DNA.
Publication details
- DOI
- 10.18295/2075-0528.2141
- Journal
- Sultan Qaboos University Medical Journal, 2(1), 11-14
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY-ND 4.0
Cite this article
APA 7
M, S., & H, A. (2025). Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction. Sultan Qaboos University Medical Journal, 2(1), 11-14. https://doi.org/10.18295/2075-0528.2141
MLA 9
M, Simsek, and Adnan H. "Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction." Sultan Qaboos University Medical Journal, vol. 2, no. 1, 2025, pp. 11-14. https://doi.org/10.18295/2075-0528.2141.
Chicago (author–date)
M, Simsek, and Adnan H. 2025. "Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction." Sultan Qaboos University Medical Journal 2 (1): 11-14. https://doi.org/10.18295/2075-0528.2141.
Harvard
M, S. and H, A. (2025) 'Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction', Sultan Qaboos University Medical Journal, 2(1), pp. 11-14. doi:10.18295/2075-0528.2141.
Vancouver
M S, H A. Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction. Sultan Qaboos University Medical Journal. 2025;2(1):11-14. doi:10.18295/2075-0528.2141
IEEE
S. M, and A. H, "Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction," Sultan Qaboos University Medical Journal, vol. 2, no. 1, pp. 11-14, 2025, doi: 10.18295/2075-0528.2141.