[
    {
        "id": "osp-11795",
        "type": "article-journal",
        "title": "Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction",
        "author": [
            {
                "family": "M",
                "given": "Simsek"
            },
            {
                "family": "H",
                "given": "Adnan"
            }
        ],
        "URL": "https://omanscience.com/en/articles/effect-of-single-mismatches-at-3-end-of-primers-on-polymerase-chain-reaction",
        "language": "en",
        "issued": {
            "date-parts": [
                [
                    2025
                ]
            ]
        },
        "container-title": "Sultan Qaboos University Medical Journal",
        "volume": "2",
        "issue": "1",
        "page": "11-14",
        "DOI": "10.18295/2075-0528.2141",
        "publisher": "Sultan Qaboos University",
        "ISSN": "2075-051X",
        "abstract": "Objective and Method – To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3•– end of a primer to amplify a 268 bp (base pair) region of the human •–globin gene using different annealing temperatures (45 to 65•C). Results – The primer with the G/T mismatch was as efficient as the normal primer (G/C match) in the amplification of a 268 bp product at all temperatures tested. However, the primers having G/A or G/G mismatches at the 3'- end did not produce any specific polymerase chain reaction (PCR) fragment at all the annealing temperatures used, except a barely detectable 268 bp product for the G/G mismatch at 45 and 50•C. Conclusion – We conclude that our PCR system was refractory to amplification when one of the primers contained a G/A or G/G mismatch at the 3•–end with template DNA."
    }
]