الباحثون

Bhuvan J. Pudashini

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مقال بحثي وصول مفتوح

Occurrence of an Old World Begomovirus Complex Infecting Radish in South of Nepal

Muhammad Shafiq Shahid, Bhuvan J. Pudashini · مجلة العلوم الزراعية والبحرية · 2025 · 10.53541/jams.vol29iss1pp71-77

Radish (Raphanus sativus) leaf curl disease (RLCD) characterized by yellowing, color breaking, and mosaic symptoms were observed in a garden at Chitwan, Southern Nepal. Polymerase chain reaction using Taq DNA polymerase followed by rolling circle amplification using φ29 polymerase identified an Old World begomovirus co …

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