الملخص
Despite growing recognition of rapid diester P turnover, quantitative data on soil DNA-P across soil types remain scarce due to methodological constraints. This study aimed to investigate the impact of changes in the analytical protocol of a soil DNA-P method developed by Paraskova et al. (2013) on DNA-P recovery and the use of the revised procedure on 32 UK soils on our understanding of DNA-P in soils. The steps of the Paraskova method were tested on two contrasting soils, and a revised version was developed and used to determine the DNA-P levels of 32 UK soils. These findings were then compared with 31P-Nuclear Magnetic Resonance (31P-NMR) speciation and other standard soil parameters for the same soils. The revised Paraskova method proved to be more cost-effective and simpler, while retaining precision and sensitivity, and DNA-P recovery could be obtained without the need for enzymes, but did require an ultrafiltration step. The DNA-P values for the 32 soils were considered small with respect to the total soil organic P. The DNA-P data obtained were significantly correlated with pH, microbial biomass P, organic matter content, and P concentration in water extracts, suggesting that the sampled DNA-P pool was associated with the living soil biota and not with stabilised soil P fractions. The modified Paraskova method can be used as an effective way to understand the biological relevance of this functionally important form of phosphorus.
الكلمات المفتاحية
بيانات النشر
- المعرّف الرقمي
- 10.53541/2410-1079.1356
- المجلة
- مجلة العلوم الزراعية والبحرية, 31(1)
- الناشر
- جامعة السلطان قابوس
- وصول مفتوح
- وصول مفتوح ذهبي
- الترخيص
- CC BY-ND 4.0
اقتبس هذه المقالة
APA 7
Massam, M. J., Menezes‐Blackburn, D., Al Kasbi, M., Wearing, C., Stutter, M. I., Giles, C. D., Darch, T. S., George, T., Shand, C. A., Lumsdon, D. G., Blackwell, M. S. A., Zhang, H., Cooper, P., Wendler, R., Brown, L. K., & Haygarth, P. M. (2026). Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils. Journal of Agricultural and Marine Sciences, 31(1). https://doi.org/10.53541/2410-1079.1356
MLA 9
Massam, Margaret J., et al. "Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils." Journal of Agricultural and Marine Sciences, vol. 31, no. 1, 2026. https://doi.org/10.53541/2410-1079.1356.
شيكاغو (المؤلف–التاريخ)
Massam, Margaret J., Daniel Menezes‐Blackburn, Mohammed Al Kasbi, Catherine Wearing, Marc I. Stutter, Courtney D. Giles, Tegan S. Darch, Timothy George, Charles A. Shand, David G. Lumsdon, Martin S. A. Blackwell, Hao Zhang, Pat Cooper, Renate Wendler, Lawrie K. Brown, and Philip M. Haygarth. 2026. "Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils." Journal of Agricultural and Marine Sciences 31 (1). https://doi.org/10.53541/2410-1079.1356.
هارفارد
Massam, M. J., Menezes‐Blackburn, D., Al Kasbi, M., Wearing, C., Stutter, M. I., Giles, C. D., Darch, T. S., George, T., Shand, C. A., Lumsdon, D. G., Blackwell, M. S. A., Zhang, H., Cooper, P., Wendler, R., Brown, L. K. and Haygarth, P. M. (2026) 'Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils', Journal of Agricultural and Marine Sciences, 31(1). doi:10.53541/2410-1079.1356.
فانكوفر
Massam MJ, Menezes‐Blackburn D, Al Kasbi M, Wearing C, Stutter MI, Giles CD, et al. Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils. Journal of Agricultural and Marine Sciences. 2026;31(1). doi:10.53541/2410-1079.1356
IEEE
M. J. Massam, D. Menezes‐Blackburn, M. Al Kasbi, C. Wearing, M. I. Stutter, C. D. Giles, T. S. Darch, T. George, C. A. Shand, D. G. Lumsdon, M. S. A. Blackwell, H. Zhang, P. Cooper, R. Wendler, L. K. Brown, and P. M. Haygarth, "Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils," Journal of Agricultural and Marine Sciences, vol. 31, no. 1, 2026, doi: 10.53541/2410-1079.1356.