[
    {
        "id": "osp-14236",
        "type": "article-journal",
        "title": "Soil DNA-Phosphorus: Method Optimisation and Application Across UK Soils",
        "author": [
            {
                "family": "Massam",
                "given": "Margaret J."
            },
            {
                "family": "Menezes‐Blackburn",
                "given": "Daniel"
            },
            {
                "family": "Al Kasbi",
                "given": "Mohammed"
            },
            {
                "family": "Wearing",
                "given": "Catherine"
            },
            {
                "family": "Stutter",
                "given": "Marc I."
            },
            {
                "family": "Giles",
                "given": "Courtney D."
            },
            {
                "family": "Darch",
                "given": "Tegan S."
            },
            {
                "family": "George",
                "given": "Timothy"
            },
            {
                "family": "Shand",
                "given": "Charles A."
            },
            {
                "family": "Lumsdon",
                "given": "David G."
            },
            {
                "family": "Blackwell",
                "given": "Martin S. A."
            },
            {
                "family": "Zhang",
                "given": "Hao"
            },
            {
                "family": "Cooper",
                "given": "Pat"
            },
            {
                "family": "Wendler",
                "given": "Renate"
            },
            {
                "family": "Brown",
                "given": "Lawrie K."
            },
            {
                "family": "Haygarth",
                "given": "Philip M."
            }
        ],
        "URL": "https://omanscience.com/ar/articles/soil-dna-phosphorus-method-optimisation-and-application-across-uk-soils",
        "language": "en",
        "issued": {
            "date-parts": [
                [
                    2026
                ]
            ]
        },
        "container-title": "Journal of Agricultural and Marine Sciences",
        "volume": "31",
        "issue": "1",
        "DOI": "10.53541/2410-1079.1356",
        "publisher": "Sultan Qaboos University",
        "ISSN": "2410-1060",
        "abstract": "Despite growing recognition of rapid diester P turnover, quantitative data on soil DNA-P across soil types remain scarce due to methodological constraints. This study aimed to investigate the impact of changes in the analytical protocol of a soil DNA-P method developed by Paraskova et al. (2013) on DNA-P recovery and the use of the revised procedure on 32 UK soils on our understanding of DNA-P in soils. The steps of the Paraskova method were tested on two contrasting soils, and a revised version was developed and used to determine the DNA-P levels of 32 UK soils. These findings were then compared with 31P-Nuclear Magnetic Resonance (31P-NMR) speciation and other standard soil parameters for the same soils. The revised Paraskova method proved to be more cost-effective and simpler, while retaining precision and sensitivity, and DNA-P recovery could be obtained without the need for enzymes, but did require an ultrafiltration step. The DNA-P values for the 32 soils were considered small with respect to the total soil organic P. The DNA-P data obtained were significantly correlated with pH, microbial biomass P, organic matter content, and P concentration in water extracts, suggesting that the sampled DNA-P pool was associated with the living soil biota and not with stabilised soil P fractions. The modified Paraskova method can be used as an effective way to understand the biological relevance of this functionally important form of phosphorus."
    }
]