Abstract
Objectives: Ionising radiation has deleterious effects on human cells. N-acetylcysteine (NAC) and cysteine, the active metabolite of NAC, are well-known radioprotective agents. Recently, a serine-magnesium sulfate combination was proposed as an antidote for organophosphate toxicity. This study aimed to investigate the use of a serine-magnesium sulfate mixture in the prevention of γ-radiation-induced DNA damage in human lymphocytes as compared to NAC and cysteine. Methods: This study was carried out at the Iran University of Medical Sciences, Tehran, Iran, between April and September 2016. Citrated blood samples of 7 mL each were taken from 22 healthy subjects. Each sample was divided into 1 mL aliquots, with the first aliquot acting as the control while the second was exposed to 2 Gy of γ-radiation at a dose rate of 102.7 cGy/minute. The remaining aliquots were separately incubated with 600 μM concentrations each of serine, magnesium sulfate, serine-magnesium sulfate, NAC and cysteine before being exposed to 2 Gy of γ-radiation. Lymphocytes were isolated using a separation medium and methyl-thiazole-tetrazolium and comet assays were used to evaluate cell viability and DNA damage, respectively. Results: The serine-magnesium sulfate mixture significantly increased lymphocyte viability and reduced DNA damage in comparison to serine, magnesium sulfate, NAC or cysteine alone (P <0.01 each). Conclusion: The findings of the present study support the use of a serine-magnesium sulfate mixture as a new, non-toxic, potent and efficient radioprotective agent.
Publication details
- DOI
- 10.18295/squmj.2016.17.02.005
- Journal
- Sultan Qaboos University Medical Journal, 17(2), 162-167
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY-ND 4.0
Cite this article
APA 7
Changizi, V., Bahrami, M., Shetab-Boushehri, M., & V., S. (2025). Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture. Sultan Qaboos University Medical Journal, 17(2), 162-167. https://doi.org/10.18295/squmj.2016.17.02.005
MLA 9
Changizi, Vahid, et al. "Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture." Sultan Qaboos University Medical Journal, vol. 17, no. 2, 2025, pp. 162-167. https://doi.org/10.18295/squmj.2016.17.02.005.
Chicago (author–date)
Changizi, Vahid, Mona Bahrami, Mahbod Shetab-Boushehri, and Seyed V. 2025. "Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture." Sultan Qaboos University Medical Journal 17 (2): 162-167. https://doi.org/10.18295/squmj.2016.17.02.005.
Harvard
Changizi, V., Bahrami, M., Shetab-Boushehri, M. and V., S. (2025) 'Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture', Sultan Qaboos University Medical Journal, 17(2), pp. 162-167. doi:10.18295/squmj.2016.17.02.005.
Vancouver
Changizi V, Bahrami M, Shetab-Boushehri M, V. S. Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture. Sultan Qaboos University Medical Journal. 2025;17(2):162-167. doi:10.18295/squmj.2016.17.02.005
IEEE
V. Changizi, M. Bahrami, M. Shetab-Boushehri, and S. V., "Prevention of γ-Radiation-Induced DNA Damage in Human Lymphocytes Using a Serine-Magnesium Sulfate Mixture," Sultan Qaboos University Medical Journal, vol. 17, no. 2, pp. 162-167, 2025, doi: 10.18295/squmj.2016.17.02.005.