Abstract
Improved and simplified HPLC conditions for the determination and quantification of both the steroidal glycoalkaloid (solamargine) and its aglycone (solasodine) are described. The best isocratic conditions were developed using a C18 column and methanol in combination with an ammonium dihydrogen phosphate buffer. The isocratic conditions were shown to be more reproducible, less time consuming and to give sharper peaks (better separation). The effects of adjusting solvent: buffer ratio, buffer pH and buffer molarity were evaluated.
Keywords
Publication details
- DOI
- 10.24200/squjs.vol18iss0pp1-10
- Journal
- Sultan Qaboos University Journal for Science, 18, 1
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY 4.0
Cite this article
APA 7
Al-Sinani, S. S., & Eltayeb, E. A. (2013). Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography. Sultan Qaboos University Journal for Science, 18, 1. https://doi.org/10.24200/squjs.vol18iss0pp1-10
MLA 9
Al-Sinani, Sana Salem, and Elsadig A. Eltayeb. "Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography." Sultan Qaboos University Journal for Science, vol. 18, 2013, pp. 1. https://doi.org/10.24200/squjs.vol18iss0pp1-10.
Chicago (author–date)
Al-Sinani, Sana Salem, and Elsadig A. Eltayeb. 2013. "Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography." Sultan Qaboos University Journal for Science 18: 1. https://doi.org/10.24200/squjs.vol18iss0pp1-10.
Harvard
Al-Sinani, S. S. and Eltayeb, E. A. (2013) 'Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography', Sultan Qaboos University Journal for Science, 18, pp. 1. doi:10.24200/squjs.vol18iss0pp1-10.
Vancouver
Al-Sinani SS, Eltayeb EA. Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography. Sultan Qaboos University Journal for Science. 2013;18:1. doi:10.24200/squjs.vol18iss0pp1-10
IEEE
S. S. Al-Sinani, and E. A. Eltayeb, "Optimised Methods for Quantitative Analysis of Solasodine and its Glycoside Solamargine by High Performance Liquid Chromatography," Sultan Qaboos University Journal for Science, vol. 18, pp. 1, 2013, doi: 10.24200/squjs.vol18iss0pp1-10.