Abstract
Objectives: This study aimed to validate pulse CO-oximetry-based haemoglobin (Hb) estimation in children and adults with thalassaemia major (TM) and to determine the impact of different baseline variables on the accuracy of the estimation. Methods: This observational study was conducted over a five-week period from March to April 2012. A total of 108 patients with TM attending the daycare thalassaemia centre of a tertiary care hospital in Muscat, Oman, were enrolled. Spot (Sp) Hb measurements were estimated using a Pronto-7® pulse CO-oximetry device (Masimo Corp., Irvine, California, USA). These were compared to venous samples of Hb using the CELL-DYN Sapphire Hematology Analyzer (Abbott Diagnostics, Abbott Park, Illinois, USA) to determine the reference (Ref) Hb levels. A multivariable linear regression model was used to assess the impact of baseline variables such as age, gender, weight, height, Ref Hb and blood pressure on the Hb estimations. Results: Of the 108 enrolled patients, there were 54 males and 54 females with a mean age of 21.6 years (standard deviation [SD] = 7.3 years; range: 2.5–38 years). The mean Ref Hb and Sp Hb were 9.4 g/dL (SD = 0.9 g/dL; range: 7.5–12.3 g/dL) and 11.1 g/dL (SD = 1.2 g/dL; range: 7.5–14.7 g/dL), respectively. The coefficient of determination (R2) was 21% with a mean difference of 1.7 g/dL (SD = 1.1 g/dL; range: −0.9–4.3 g/dL). In the multivariable model, the Ref Hb level (P = 0.001) was the only statistically significant predictor. Conclusion: The Pronto-7® pulse CO-oximetry device was found to overestimate Hb levels in patients with TM and therefore cannot be recommended. Further larger studies are needed to confirm these results.
Publication details
- DOI
- 10.18295/2075-0528.1620
- Journal
- Sultan Qaboos University Medical Journal, 14(4), 468-472
- Publisher
- Sultan Qaboos University
- Open access
- Gold open access
- License
- CC BY-ND 4.0
Cite this article
APA 7
Al-Khabori, M. K., Al-Riyami, A. Z., Al-Huneini, K., Mohammed, Al-Hashim, A. H., Al-Kemyani, N., Alqarshoubi, I., Khan, H., Al-Amrani, K., & Daar, S. (2025). Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major. Sultan Qaboos University Medical Journal, 14(4), 468-472. https://doi.org/10.18295/2075-0528.1620
MLA 9
Al-Khabori, Murtadha K., et al. "Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major." Sultan Qaboos University Medical Journal, vol. 14, no. 4, 2025, pp. 468-472. https://doi.org/10.18295/2075-0528.1620.
Chicago (author–date)
Al-Khabori, Murtadha K., Arwa Z. Al-Riyami, Khalil Al-Huneini, Mohammed, Abdul Hakeem Al-Hashim, Nasser Al-Kemyani, Issa Alqarshoubi, Hammad Khan, Khalfan Al-Amrani, and Shahina Daar. 2025. "Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major." Sultan Qaboos University Medical Journal 14 (4): 468-472. https://doi.org/10.18295/2075-0528.1620.
Harvard
Al-Khabori, M. K., Al-Riyami, A. Z., Al-Huneini, K., Mohammed, Al-Hashim, A. H., Al-Kemyani, N., Alqarshoubi, I., Khan, H., Al-Amrani, K. and Daar, S. (2025) 'Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major', Sultan Qaboos University Medical Journal, 14(4), pp. 468-472. doi:10.18295/2075-0528.1620.
Vancouver
Al-Khabori MK, Al-Riyami AZ, Al-Huneini K, Mohammed, Al-Hashim AH, Al-Kemyani N, et al. Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major. Sultan Qaboos University Medical Journal. 2025;14(4):468-472. doi:10.18295/2075-0528.1620
IEEE
M. K. Al-Khabori, A. Z. Al-Riyami, K. Al-Huneini, Mohammed, A. H. Al-Hashim, N. Al-Kemyani, I. Alqarshoubi, H. Khan, K. Al-Amrani, and S. Daar, "Non-Invasive Haemoglobin Estimation in Patients with Thalassaemia Major," Sultan Qaboos University Medical Journal, vol. 14, no. 4, pp. 468-472, 2025, doi: 10.18295/2075-0528.1620.