Abstract
Background: Due to numerous side effects of common drugs in treatment of leishmaniasis, new therapeutic approaches focus on herbal compounds. Therefore, we aimed to determine the effect of crocin and stigmasterol on in-vitro growth of promastigotes and amastigotes of Leishmania major in the Department of Parasitology, Pasteur Institute, Tehran, Iran in 2018. Methods: The effect of different concentrations of crocin and stigmasterol were evaluated by determining their in-vitro inhibitory effects on promastigotes and amastigotes of the L. major using MTT assay. Results: The fatality rate was 65.27% and 71.96% for crocin and stigmasterol respectively at 24 h post-culture in concentration of 50 μg/mL. The mean inhibitory effect of crocin and stigmasterol on L major amastigotes after 72 h were 52.22% and 38.96%. Conclusion: The crocin and stigmasterol had efficient adverse effects on promastigote and amastigotes of L. major, hence, further studies on the anti-leishmanial effects of these herbal compounds in human and animal models are recommended.
Keywords
Subject
Publication details
- DOI
- 10.18502/ijpa.v16i1.5535
- Journal
- Iranian Journal of Parasitology, 16(1), 151-158
- Publisher
- Tehran University of Medical Sciences
- Open access
- Gold open access
- License
- CC BY-NC 4.0
Cite this article
APA 7
Ranjbar, R., Shayanfar, P., & Maniati, M. (2021). In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER). Iranian Journal of Parasitology, 16(1), 151-158. https://doi.org/10.18502/ijpa.v16i1.5535
MLA 9
Ranjbar, Reza, et al. "In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER)." Iranian Journal of Parasitology, vol. 16, no. 1, 2021, pp. 151-158. https://doi.org/10.18502/ijpa.v16i1.5535.
Chicago (author–date)
Ranjbar, Reza, Peyman Shayanfar, and Mahmood Maniati. 2021. "In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER)." Iranian Journal of Parasitology 16 (1): 151-158. https://doi.org/10.18502/ijpa.v16i1.5535.
Harvard
Ranjbar, R., Shayanfar, P. and Maniati, M. (2021) 'In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER)', Iranian Journal of Parasitology, 16(1), pp. 151-158. doi:10.18502/ijpa.v16i1.5535.
Vancouver
Ranjbar R, Shayanfar P, Maniati M. In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER). Iranian Journal of Parasitology. 2021;16(1):151-158. doi:10.18502/ijpa.v16i1.5535
IEEE
R. Ranjbar, P. Shayanfar, and M. Maniati, "In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of Leishmania major (MHOM/IR/75/ER)," Iranian Journal of Parasitology, vol. 16, no. 1, pp. 151-158, 2021, doi: 10.18502/ijpa.v16i1.5535.