Abstract
One of the most common malignancies in women is breast cancer. β-escin has pharmacological anticancer effects. 5-fluorouracil (5-FU) has antimetabolite and antiproliferative properties. The purpose of this study was to investigate the combined effects of 5-FU and β-escin on apoptosis, colony formation, Bcl-2 signaling protein, and p53 gene expression in MCF7 breast cancer cell line. The cytotoxic effects, the number of colonies, apoptosis, p53 gene expression, and Bcl-2 signaling protein of the combined 5-FU and β-escin on MCF7 cells were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and western blotting methods, respectively. Half-maximal inhibitory concentration values of β-escin and 5-FU were 80 μg/ml and 2 μM, respectively. The combination of 5-FU and β-escin on MCF7 cell viability showed a combination index equal to 0.5. The expression of p53 and apoptosis increased in the combination of 5-FU and β-escin on MCF7 cells compared to that of control group (P < 0.05). In addition, the number of colonies and Bcl-2 signaling protein in combination of 5-FU and β-escin decreased with respect to untreated control cells or single treatment of 5-FU and β-escin. The combination of 5-FU and β-escin not only has synergistic effects by increasing cell apoptosis and p53 gene expression but also decreases Bcl-2 signaling protein in MCF7 cell lines.
Keywords
Subject
Publication details
- DOI
- 10.4103/jmss.jmss_44_18
- Journal
- Journal of Medical Signals and Sensors, 9(3), 196
- Publisher
- Wolters Kluwer Medknow Publications
- Open access
- Gold open access
- License
- CC BY-NC-SA 4.0
Cite this article
APA 7
Heidarian, E., Mazrouei, R., Raeisi, E., & Lemoigne, Y. (2019). Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells. Journal of Medical Signals and Sensors, 9(3), 196. https://doi.org/10.4103/jmss.jmss_44_18
MLA 9
Heidarian, Esfandiar, et al. "Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells." Journal of Medical Signals and Sensors, vol. 9, no. 3, 2019, pp. 196. https://doi.org/10.4103/jmss.jmss_44_18.
Chicago (author–date)
Heidarian, Esfandiar, Raziyeh Mazrouei, Elham Raeisi, and Yves Lemoigne. 2019. "Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells." Journal of Medical Signals and Sensors 9 (3): 196. https://doi.org/10.4103/jmss.jmss_44_18.
Harvard
Heidarian, E., Mazrouei, R., Raeisi, E. and Lemoigne, Y. (2019) 'Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells', Journal of Medical Signals and Sensors, 9(3), pp. 196. doi:10.4103/jmss.jmss_44_18.
Vancouver
Heidarian E, Mazrouei R, Raeisi E, Lemoigne Y. Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells. Journal of Medical Signals and Sensors. 2019;9(3):196. doi:10.4103/jmss.jmss_44_18
IEEE
E. Heidarian, R. Mazrouei, E. Raeisi, and Y. Lemoigne, "Activation of p53 gene expression and synergistic antiproliferative effects of 5-fluorouracil and β-escin on MCF7 cells," Journal of Medical Signals and Sensors, vol. 9, no. 3, pp. 196, 2019, doi: 10.4103/jmss.jmss_44_18.