الملخص
Background: Epidemiological studies, classification and genetic studies of Leishmania species are effective in treatment, control and prevention in endemic areas. We aimed to investigate the genetic diversity and phylogeny of Leishmania in Zoonotic foci located in northeastern Iran using nagt gene for the first time. Methods: DNA of 100 confirmed positive slides collected from the health centers of Sarkhes, Darghez, Fariman, Esfarayen, and Sabzevar were extracted during 2020-2021. The partial sequence of kDNA was amplified to identify the species. Twenty-five DNA samples were randomly subjected to amplify by nagt gene primes and were sequenced. The sequences were aligned with reference sequences in National Center for Biotechnology Information (NCBI). Then, the genetic similarities of the sequences were checked using Clustalx2.1 software and the phylogenetic tree was drawn by Mega 7 software. Results: All the positive samples were diagnosed as L. major. Approximately, half of the sequences of species were similar to two reference genes JX103550.1:404-712 L. major Esfahan and KX759012.1:568-807 L. Major Ilam (more than 90% similarity). According to the results of the phylogeny tree, the closest genotype to our study samples was JX103550.1:404-712 L. major Esfahan. Conclusion: The most causative agent CL in these areas was L. major. The genetic diversity of L. major was high such as other zoonotic foci in Iran. Due to the high similarity of the strains in the study areas with the strains of Isfahan and Ilam, similar control and prevention methods is suggested in these areas.
الكلمات المفتاحية
الموضوع
بيانات النشر
- المعرّف الرقمي
- 10.18502/ijpa.v18i3.13752
- المجلة
- Iranian Journal of Parasitology, 18(3), 294-300
- الناشر
- Tehran University of Medical Sciences
- وصول مفتوح
- وصول مفتوح ذهبي
- الترخيص
- CC BY-NC 4.0
اقتبس هذه المقالة
APA 7
Shamsian, S. A., Mirizadeh, H., Sangani, G. S., Zarean, M., Berenji, F., Najjari, M., Fata, A., Serki, E., Tahreian, V., & Farash, B. R. H. (2023). Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran. Iranian Journal of Parasitology, 18(3), 294-300. https://doi.org/10.18502/ijpa.v18i3.13752
MLA 9
Shamsian, Seyed Aliakbar, et al. "Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran." Iranian Journal of Parasitology, vol. 18, no. 3, 2023, pp. 294-300. https://doi.org/10.18502/ijpa.v18i3.13752.
شيكاغو (المؤلف–التاريخ)
Shamsian, Seyed Aliakbar, Hamed Mirizadeh, Ghodratollah Salehi Sangani, Mahdi Zarean, Fariba Berenji, Mohsen Najjari, Abdolmajid Fata, Elham Serki, Vahideh Tahreian, and Bibi Razieh Hosseini Farash. 2023. "Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran." Iranian Journal of Parasitology 18 (3): 294-300. https://doi.org/10.18502/ijpa.v18i3.13752.
هارفارد
Shamsian, S. A., Mirizadeh, H., Sangani, G. S., Zarean, M., Berenji, F., Najjari, M., Fata, A., Serki, E., Tahreian, V. and Farash, B. R. H. (2023) 'Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran', Iranian Journal of Parasitology, 18(3), pp. 294-300. doi:10.18502/ijpa.v18i3.13752.
فانكوفر
Shamsian SA, Mirizadeh H, Sangani GS, Zarean M, Berenji F, Najjari M, et al. Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran. Iranian Journal of Parasitology. 2023;18(3):294-300. doi:10.18502/ijpa.v18i3.13752
IEEE
S. A. Shamsian, H. Mirizadeh, G. S. Sangani, M. Zarean, F. Berenji, M. Najjari, A. Fata, E. Serki, V. Tahreian, and B. R. H. Farash, "Molecular Identification and Phylogenetic Analysis of Leishmania major Isolated from Zoonotic Cutaneous Leishmaniasis Foci in Northeast Iran," Iranian Journal of Parasitology, vol. 18, no. 3, pp. 294-300, 2023, doi: 10.18502/ijpa.v18i3.13752.