الملخص
Background: We described here an aptamer-based magnetic nanoprobe for measuring the amount of chloramphenicol (CAP) in milk. Methods: The nanoprobe presented in this method consists of a magnetic nanoparticle conjugated to a specific CAP aptamer. If the target is detected in the sample, the nanoprobe binds to it, and the aptamer forms a G-quadruplex structure. This structure mimics the peroxidase activity in the presence of the hemin cofactor. If tetramethylbenzidine is added to the sample containing the nanoprobe, a blue color light is observed. After adding a stop reagent solution, the color produced is measured by a microplate reader and a portable meter. Results: This study proves a 99% positive linear relationship between the microplate reader's results and the portable meter results. Conclusion: Conjugation of the aptamer to magnetic nanoparticles and applying magnetic separation operations change the nanoprobe performance by 11% for both mentioned devices.
الكلمات المفتاحية
الموضوع
بيانات النشر
- المعرّف الرقمي
- 10.4103/jmss.jmss_177_21
- المجلة
- Journal of Medical Signals and Sensors, 13(2), 136-143
- الناشر
- Wolters Kluwer Medknow Publications
- وصول مفتوح
- وصول مفتوح ذهبي
- الترخيص
- CC BY-NC-SA 4.0
اقتبس هذه المقالة
APA 7
Marofi, H., Rafati, A., & Gill, P. (2023). Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader. Journal of Medical Signals and Sensors, 13(2), 136-143. https://doi.org/10.4103/jmss.jmss_177_21
MLA 9
Marofi, Hossein, et al. "Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader." Journal of Medical Signals and Sensors, vol. 13, no. 2, 2023, pp. 136-143. https://doi.org/10.4103/jmss.jmss_177_21.
شيكاغو (المؤلف–التاريخ)
Marofi, Hossein, Adele Rafati, and Pooria Gill. 2023. "Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader." Journal of Medical Signals and Sensors 13 (2): 136-143. https://doi.org/10.4103/jmss.jmss_177_21.
هارفارد
Marofi, H., Rafati, A. and Gill, P. (2023) 'Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader', Journal of Medical Signals and Sensors, 13(2), pp. 136-143. doi:10.4103/jmss.jmss_177_21.
فانكوفر
Marofi H, Rafati A, Gill P. Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader. Journal of Medical Signals and Sensors. 2023;13(2):136-143. doi:10.4103/jmss.jmss_177_21
IEEE
H. Marofi, A. Rafati, and P. Gill, "Aptamer-Based Magnetic Nanoprobe for Quantitative Measurement of Chloramphenicol in Milk through Portable Reader," Journal of Medical Signals and Sensors, vol. 13, no. 2, pp. 136-143, 2023, doi: 10.4103/jmss.jmss_177_21.