[
    {
        "id": "osp-3421",
        "type": "article-journal",
        "title": "Molecular Characterization of MDR and XDR Clinical Strains from a Tertiary Care Center in North India by Whole Genome Sequence Analysis",
        "author": [
            {
                "family": "Tayyaba",
                "given": "Uzma"
            },
            {
                "family": "Khan",
                "given": "Shariq Wadood"
            },
            {
                "family": "Sultan",
                "given": "Asfia"
            },
            {
                "family": "Khan",
                "given": "Fatima"
            },
            {
                "family": "Akhtar",
                "given": "Anees"
            },
            {
                "family": "Nagaraj",
                "given": "Geetha"
            },
            {
                "family": "Ahmed",
                "given": "Shariq"
            },
            {
                "family": "Bhattacharya",
                "given": "Bhaswati"
            }
        ],
        "URL": "https://omanscience.com/en/articles/molecular-characterization-of-mdr-and-xdr-clinical-strains-from-a-tertiary-care-center-in-north-india-by-whole-genome-sequence-analysis",
        "language": "en",
        "issued": {
            "date-parts": [
                [
                    2024
                ]
            ]
        },
        "container-title": "Journal of the Oman Medical Association",
        "volume": "1",
        "issue": "1",
        "page": "29-47",
        "DOI": "10.3390/joma1010005",
        "publisher": "MDPI",
        "abstract": "Whole genome sequencing (WGS) has the potential to greatly enhance AMR (Anti-microbial Resistance) surveillance. To characterize the prevalent pathogens and dissemination of various AMR-genes, 73 clinical isolates were obtained from blood and respiratory tract specimens, were characterized phenotypically by VITEK-2 (bioMerieux), and 23 selected isolates were genotypically characterized by WGS (Illumina). AST revealed high levels of resistance with 50.7% XDR, 32.9% MDR, and 16.4% non-MDR phenotype. A total of 11 K. pneumoniae revealed six sequence types, six K-locus, and four O-locus types, with ST437, KL36, and O4 being predominant types, respectively. They carried ESBL genes CTX-M-15 (90.9%), TEM-1D (72.7%), SHV-11 (54.5%), SHV-1, SHV-28, OXA-1, FONA-5, and SFO-1; NDM-5 (72.7%) and 63.6%OXA48-like carbapenamases; 90.9%OMP mutation; dfrA12, sul-1, ermB, mphA, qnrB1, gyrA831, and pmrB1 for other groups. Virulence gene found were Yerisiniabactin (90.9%), aerobactin, RmpADC, and rmpA2. Predominant plasmid replicons were Col(pHAD28), IncFII, IncFIB(pQil), and Col440. A total of seven XDR A. baumannii showed single MLST type(2) and single O-locus type(OCL-1); with multiple AMR-genes: blaADC-73, blaOXA-66, blaOXA-23, blaNDM-1, gyrA, mphE, msrE, and tetB. Both S. aureus tested were found to be ST22, SCCmec IVa(2B), and spa type t309; multiple AMR-genes: blaZ, mecA, dfrC, ermC, and aacA-aphD. Non-MDR Enterococcus faecalis sequenced was ST 946, with multiple virulence genes. This study documents for the first-time prevalent virulence genes and MLST types, along with resistance genes circulating in our center."
    }
]