[
    {
        "id": "osp-11231",
        "type": "article-journal",
        "title": "Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture",
        "author": [
            {
                "family": "Al-Jasmi",
                "given": "Fatma"
            },
            {
                "family": "Pramathan",
                "given": "Thachillath"
            },
            {
                "family": "Sahari",
                "given": "Adnan"
            },
            {
                "family": "Bahjat",
                "given": ""
            },
            {
                "family": "Penefsky",
                "given": "Harvey S."
            },
            {
                "family": "Souid",
                "given": "Abdul-Kader"
            }
        ],
        "URL": "https://omanscience.com/en/articles/mitochondrial-oxygen-consumption-by-the-foreskin-and-its-fibroblast-rich-culture",
        "language": "en",
        "issued": {
            "date-parts": [
                [
                    2025
                ]
            ]
        },
        "container-title": "Sultan Qaboos University Medical Journal",
        "volume": "13",
        "issue": "3",
        "page": "411-416",
        "DOI": "10.18295/2075-0528.1497",
        "publisher": "Sultan Qaboos University",
        "ISSN": "2075-051X",
        "abstract": "Objectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures.Methods: Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Results: In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confirming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confirming that the oxidation occurred mainly in the mitochondrial respiratory chain. The rate of foreskin respiration (mean ± SD) was 0.074 ± 0.02 μM O2 min-1 mg-1 (n = 23). The corresponding rate for fibroblast-rich cultures was 9.84 ± 2.43 μM O2 min-1 per 107 cells (n = 15). Fibroblast respiration was significantly lower in a male infant with dihydrolipoamide dehydrogenase gene mutations, but normalised with the addition of thiamine or carnitine. Conclusion: The foreskin and its fibroblast-rich culture are suitable for assessment of cellular respiration. However, the clinical utility of foreskin specimens to detect disorders of impaired cellular bioenergetics requires further investigation."
    }
]